Influenza peptide M1 (56-66) has become important for research, especially in the area of vaccine development.  It contains nine amino acids.  The peptide is very hydrophobic - there is only one hydroxy group, one amino group, and one carboxylic group.  Although it is not complicated to attach those amino acids by solid phase synthesis, the purification in of the crude peptide rp-HPLC is difficult due to its poor solubility.  Even if it can be loaded to the column, the yield is very low.  However, the purification of this particular peptide is necessary to have bioactivities.  Crude peptide does not provide good results.  

Sequence: M1 (58-66)

[H]- Gly-Ile-Leu-Gly-Phe-Val-Phe-Thr-Leu -[OH]

Purity: > 90% by HPLC

Package: 5 mg, 20 mg, 50 mg, 100 mg

References:

1. Identification of viral molecules recognized by influenza specific human cytotoxic T lymphocytes. F Gotch et al. J Ex Med 1987, 165. 408-416. (Good description of the Influenza A proteins).

2. Cytotoxic T lymphocytes recognize a fragmento of influenza A virus matrix protein in association with HLA-A2. F Gotch, J Rothbard, K Howland, A Townsend, and A Mc Michael. 1987. Nature. (first description of the peptide)

3. The minimun peptide epitope from influenza virus matrix protein.  MA Bednarek et al. J. Immunology 1991, 147, 4047-4053.

4. The antigenic identity of peptide MHC complexes: a comparison of the conformations of five viral peptides presented by HLA-A2.  Madden et al. 1993, Cell, 75: 693-708.  (the crystal structure of A2 with the peptide).

 

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