Storage    

    For long term storage, peptides should be stored in dry powder at -78° C.  If peptides are in solution, it should be kept frozen at -78° C  for maximum stability.  Peptides may be also stored at -20^o C (freezer) for short term usage.  Most peptides containing Trp, Met, Cys, Asn or Gln have a limited lifetime. Long-term storage is not recommended.

Back to Room Temperature

    Allow peptide samples to come to room temperature in a desiccator prior to opening the peptide sample bottle.  Weigh out peptides quickly and reseal bottle tightly.

Dissolution

    Dissolve peptides in an appropriate buffer.  An acidic peptide is easy to dissolve in basic buffer and a  basic peptide is easy to dissolve in acidic buffer.  However, Cysteine in peptide may form a disulfide bridge, either intra- or intermolecular, in a high pH solution.   If necessary, the solution can be sonicated briefly.  Peptides containing Trp, Met, or Cys require special care to avoid oxidation. Oxygen-free water or reducing agents can be used.

 

 

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