Introduction

      Fluorescent peptides and other dye-bonded peptides are important tools in biochemical and biological research to study interactions between proteins, to analyze conformational structures, to design substrates, etc.  The most important characteristics of fluorescent peptides are high sensitivity and non-radioactive detection.  Many commercial protein kinase assays have been developed using fluorescent peptides.   Princeton BioMolecules has developed an unique method to synthesize fluorescent peptides and other dye-bonded peptides with different specific requirements. 

Properties

    A fluorescent dye can be attached to a peptide at a specific point through a covalent bond depending on the sequence of peptide.  Fluorecein is derivated at either 5- or 6- position with a carboxylic acid, which is able to be activated for the reaction with an amino group in the peptide part.  The following is some information about fluorescent peptides. If you would like to discuss with us on the chemistry and applications, please contact us. 

1. The choice of dyes:   

Fluorescein (yellow), 
Tetramethylrhodamine (purple), 
Sulforhodamine (red), 
Dinitrophenyl (yellow), 
Dansyl (yellow),
etc.

2. The linkage between dye and peptide is a covalent bond, which is stable and not destructive under most biological conditions.

3. A functional linker can be introduced between dye and peptide.

4. A dye can be at any position: N-terminus, C-terminus, or in the middle of sequence.

5. Additional dyes, linkers, or covalent bonds with specified chemical structures can be developed at your request.

Examples

The following are some examples of dye-bonded peptides synthesized at Princeton BioMolecules:

1. [Fluorescein]-LC- Asp-Glu-Val-Asp-Lys -[NH2]

2. [H]- Asp-Glu-Val-Asp-Lys(fluorescein)- [NH2]

3. [Tetramethylrhodamine]- Asp-Glu-Val-Asp-Lys -[NH2]

4. [H]- Asp-Glu-Val-Asp-Lys(tetramethylrhodamine)- [NH2]

5. [H]- Phe-Leu-Pro-Ser-Asp-Cys(fluorescein)-Phe-Pro-Ser-Val- [NH2]

6. [Fluorescein]- Thr-Arg-Asp-Ile-Tyr(phospho)-Glu-Thr-Asp-Tyr-Tyr-Arg- [OH]

7. [Dinitrophenyl]- Ser-Pro-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-[NH2]

 

 Back to Home Page        Contact Us