Introduction

    Dye peptide is prepared by attaching a dye to a peptide at a specific point through a covalent bond.  The position of the linkage is depend on the chemical structure of dye and sequence of peptide.  Usually suitable functional groups should be available on both dye and peptide to form a chemical bond.  The following is some information about dye peptides. If you would like to discuss with us on the chemistry and applications, please contact us.  

Fluorescent Peptides 

    Fluorescent peptides and other dye-bonded peptides are important tools in biochemical and biological research to study interactions between proteins, to analyze conformational structures, to design substrates, etc.  The most important characteristics of fluorescent peptides are high sensitivity and non-radioactive detection.  Many commercial protein kinase assays have been developed using fluorescent peptides.   Princeton BioMolecules has developed an unique method to synthesize fluorescent peptides and other dye-bonded peptides with different specific requirements. 

1. The choice of dyes:   

Fluorescein (yellow), 
Tetramethylrhodamine (purple), 
Sulforhodamine (red), 
Dinitrophenyl (yellow), 
Dansyl (yellow),
etc.

2. The linkage between dye and peptide is a covalent bond, which is stable and not destructive under most biological conditions.

3. A functional linker can be introduced between dye and peptide.

4. A dye can be at any position: N-terminus, C-terminus, or in the middle of sequence.

5. Additional dyes, linkers, or covalent bonds with specified chemical structures can be developed at your request.

Examples

The following are some examples of dye-bonded peptides synthesized at Princeton BioMolecules:

1. [Fluorescein]-LC- Asp-Glu-Val-Asp-Lys -[NH2]

2. [H]- Asp-Glu-Val-Asp-Lys(fluorescein)- [NH2]

3. [Tetramethylrhodamine]- Asp-Glu-Val-Asp-Lys -[NH2]

4. [H]- Asp-Glu-Val-Asp-Lys(tetramethylrhodamine)- [NH2]

5. [H]- Phe-Leu-Pro-Ser-Asp-Cys(fluorescein)-Phe-Pro-Ser-Val- [NH2]

6. [Fluorescein]- Thr-Arg-Asp-Ile-Tyr(phospho)-Glu-Thr-Asp-Tyr-Tyr-Arg- [OH]

7. [Dinitrophenyl]- Ser-Pro-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-[NH2]

 

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