Introduction
Antibody is
important in medical research and medical industry. It is one of the most
important tools for the diagnosis and components for the vaccine. Peptide
can be used as an antigen if it is conjugated to a big protein as a
carrier. Anti-peptide polyclonal antiserum production has become more
important in our services. Followings are some information of our services
in this area.
Antigenicity
Prediction
Some
peptide sequences are antigenic, some are not. It is
difficult to predict with 100% accuracy, because
anitgenicity is a very complicate issue. However many
theories have been developed and many software programs are
available to predict antigenicity of peptide
sequences. PBC has used a program for the service of
this prediction. It has been very much successful and
many of our customers are quite satisfied. If you like
us to provide this service, you may visit our Antigenicity
Prediction page.
Synthesis of Peptides
Basically there are two kinds of peptides to use as an antigen. First kind is
regular peptide which is synthesized and purified by HPLC to greater than 80%
and 20 mg with QC of HPLC and MS. Second kind is MAP peptide which is prepared
on a Lysine core of 20 mg with QC of HPLC.
Conjugation of Peptides
MAP peptide does not need to conjugate to a carrier protein to be used as
an antigen. However, conjugation is necessary for a small regular peptide. A
peptide is conjugated to a carrier protein via a stable covalent bond: one
side of the linker attaches to the protein through an amino group, the other
side attaches to the peptide through an amino or a sulfhydryl group. The
spacer between peptide and carrier protein is a group of six methylenes, -CH6-
extended for a distance of 7.5 A, which allows the peptide more accessibility
by sticking out from the area of carrier protein. The straight carbon chain
also produces a clean background. The site for conjugation can be at the N- or
C-terminal, or any point in the sequence of peptide. The conjugated product is
delivered in a form of lyophilized power, which is easy to reconstitute.
Immunization Protocol
Day
Procedure
01 Two
NZW female rabbits
01 Prebleed , Primary
SC/ID: 250ug with FCA
14 1st boost SC/IM:
250ug with FIA
28 2nd boost SC/IM:
250ug with FIA
42 3nd boost SC/IM:
250ug with FIA
52 1st production bleed
56 4nd boost SC/IM:
250ug with FIA
56 Shipping prebleed and 1st prod. bleed
66 2nd production bleed
70 Terminated bleed and exsanguination
70 Shipping 2nd prod. & terminated bleed
The above schedule may be
extended with necessary procedures, such as boosts, bleeds, etc., to receive the
best results according to customer's needs.
PBC also provides services to produce antiserum according to customer’s
specified protocol.
Technical Information
PBC technical support group likes to discuss with customers about the
specifications of peptides and antisera. We also provide information about the
design of a peptide for antiserum production, such as antigenicities,
synthetic difficulties, hydrophobic index, and others. For more information,
please either visit our Tech Info
page.
Back to
Home Page
Contact Us
